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Objective:To investigate the expression and clinical significance of peripheral blood miRNA-146a, miRNA-155 and miRNA-186 in patients with severe pneumonia.Methods:Seventy-nine patients with severe pneumonia who received treatment in Eastern Branch of Ningbo Medical Center Li Huili Hospital from February 2019 to February 2021 were included in this study. They were divided into survival and death groups according to prognosis at 28 days after admission. An additional 60 subjects who concurrently received health examination in the same hospital were included in the control group. Peripheral blood samples were collected and serum was separated. The expression of miRNA-146a, miRNA-155 and miRNA-186 in peripheral blood was determined by real-time fluorescent quantitative PCR.Results:Acute Physiology and Chronic Health Evaluation II (APACHE II) score in the severe pneumonia group was significantly higher than that in the control group [(18.54 ± 2.83) points vs. (3.18 ± 0.57) points, t = 41.37, P < 0.05]. The relative expression of miRNA-146a in peripheral blood in the severe pneumonia group was significantly lower than that in the control group [(0.32 ± 0.07) vs. (1.08 ± 0.21), while the relative expression of miRNA-155 and miRNA-186 in the severe pneumonia group were (2.54 ± 0.46) and (3.16 ± 0.38), which were significantly higher than (0.42 ± 0.09) and (0.89 ± 0.17) in the control group ( t = 30.07, 35.16, 43.08, all P < 0.05). The relative expression of miRNA-146a in peripheral blood in the death group was significantly lower than that in the survival group [(0.25 ± 0.68) vs. (0.59 ± 0.12), t = 19.11, P < 0.001]. The relative expression of miRNA-155 and miRNA-186 in the death group were (3.97 ± 0.78) and (5.23 ± 0.86), which were significantly higher than (0.89 ± 0.21) and (1.52 ± 0.23) in the survival group ( t = 27.69, 30.29, both P < 0.05). APACHE II score was linearly negatively correlated with miRNA-146a ( r = -0.75, P = 0.015), and it was linearly positively correlated with miRNA-155 and miRNA-186 ( r = 0.82, 0.70, P = 0.002, 0.021). In the diagnosis of severe pneumonia, miRNA-146a has a sensitivity of 72.34% and a specificity of 62.50%; miRNA-155 has a sensitivity of 75.00% and a specificity of 62.96%; miRNA-186 has a sensitivity of 66.67% and a specificity of 48.65%. Conclusion:Peripheral blood miRNA-146a is lowly expressed in severe pneumonia, while miRNA-155 and miRNA-186 are highly expressed in severe pneumonia, and miRNA-146a, miRNA-155 and miRNA-186 are closely related to the prognosis of severe pneumonia.
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AIM: To establish the quality standards for Chinese extractum angelicae liquidum. METHODS: TLC was used to identify ferulic acid and ligustilide and HPLC to determine the content of ferulic acid and ligustil-ide. HPLC was performed on a Diamonsil ODS-C_(18) analytical column(250 mm×4.6 mm, 5 μm) with gradient elu-tion(0-15 min, 38%A; 15-20 min, 38%A-70%A; 20-40 min, 70%A; 40-45 min, 70%A-38%A) of methanol (A, containing 0.4% glacial acetic) and 0.4% glacial acetic acid(B) at the flow rate of 1.0 mL/min. The diode array detection wavelength was set at 323 nm and the column temperature was at 35℃. RESULTS: The linear range of ferulic acid and ligustilide were from 1.008 μg/mL to 10.08 μg/mL and 9.985 μg/mL to 99.85 μg/mL,the average recoveries of both were 98.11% and 101.61%, RSD were 1.58% and 1.32%. CONCLUSION: The method is rapid, simple and accurate with high reproducibility and can be used to control the quality of Chinese extractum angelicae liquidum.
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AIM : To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS: Using Diamonsil C_(18) (4.6 mm×250 mm,5 μm) as analytical column.The mobile phase consisted of acetonitrile (A) and 0.1% phosphoric acid ( B ) with gradient elution : 0～18rain,83%～80% B; 18～30 min,80%～86% B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min; Column temperature was at 35 ℃.RESULTS: A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00～48.00 μg/mL( r =0.999 3 ) and 0.64 ～ 40.72 μg/mL( r = 0.999 8 ),respectively.CONCLUSION : The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.
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OBJECTIVE: To improve the dissolution rate of nitrendipine in vitro using co-grinding method.METHODS: Single-factor test was adopted to detect effect of phases of co-grinding,category of excipients (MCC,PVPk30,HPC,HPMC),time (0,10,20,30,40,50,60 min) of co-grinding and ratio of principal component to excipients (1 ∶ 1,1 ∶ 2,1 ∶ 3,1 ∶ 4,1 ∶ 5,1 ∶ 6,1 ∶ 7,1 ∶ 8,1 ∶ 9) on in vitro dissolution of nitrendipine power and tablet.RESULTS: The condition of co-grinding method was as follows: dual co-grinding phase,HPC or MCC as excipients,co-grinding time of 40 min,ratio of principal component to excipients was 1 ∶ 4.Accumulative dissolution rate of nitrendipine powder was more than 80% within 10 min and that of nitrendipine tablet was more than 80% within 40 min.CONCLUSION: Co-grinding method can improve the dissolution rate of poorly water-soluble nitrendipine in vitro under suitable condition.
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Objective To prepare osmotic pump-controlled release tablets of total flavones in Lysimachia clethroides.Methods Two components of the extract from L.clethroides,rutin and naringenin-7-O-glucoside were used to evaluate the release behavior of osmotic pump controlled release tablets.Single factor investigation was carried out on the membrane compositions and orifice variables,and uniform design was used to optimize the formulation of coating mambrane.Results The membrane weight,PEG400 content,and dibutyl phthalote(DBP) content were the main factors influencing the drug release,and based on 45% and 8.5% of cellulose acetate,respectively,to prepare osmotic pump-controlled release tablets could achieve the desired zero-order release profile.Conclusion The formulation and technology are simple and easy to be carried out.Osmotic pump-controlled release tablets have a stable drug release bahavior and a good reproducibility.
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AIM: To establish the quality standards for Chinese extractum angelicae liquidum. METHODS: TLC was used to identify ferulic acid and ligustilide and HPLC to determine the content of ferulic acid and ligustilide.HPLC was performed on a Diamonsil ODS-C_18 analytical column(250 mm?4.6 mm,5 ?m) with gradient elution(0-15 min,38%A;15-20 min,38%A70%A;20-40 min,70%A;40-45 min,70%A-38%A) of methanol(A,containing 0.4% glacial acetic) and 0.4% glacial acetic acid(B) at the flow rate of 1.0 mL/min.The diode array detection wavelength was set at 323 nm and the column temperature was at 35 ℃. RESULTS: The linear range of ferulic acid and ligustilide were from 1.008 ?g/mL to 10.08 ?g/mL and 9.985 ?g/mL to 99.85 ?g/mL,the average recoveries of both were 98.11% and 101.61%,RSD were 1.58% and 1.32%.CONCLUSION: The method is rapid,simple and accurate with high reproducibility and can be used to control the quality of Chinese extractum angelicae liquidum.
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AIM:To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS:Using Diamonsil C_ 18(4.6 mm ?250 mm,5?m)as analytical column.The mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid(B)with gradient elution:0~18 min,83%~80%B;18~30 min,80%~86%B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min;Column temperature was at 35 ℃.RESULTS :A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00~48.00 ?g/mL(r=0.999 3)and 0.64~40.72 ?g/mL(r=0.999 8),respectively.CONCLUSION:The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.